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anti atg8  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti atg8
    Anti Atg8, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/autophagy+antibody+sampler+kit/Autophagy+Atg8+Family+Antibody+Sampler+Kit/bio_rxiv__64898__2026__03__23__713835-188-29-30
    Average 94 stars, based on 6 article reviews
    anti atg8 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Polyprenylated Acylphloroglucinols from Hypericum rochelii and Hypericum olympicum —Cytotoxic Effects on Non-Tumorigenic Cell Lines and Antibacterial Potential
    Article Snippet: The separated proteins were transferred to a polyvinylidene difluoride (PVDF) membrane, a part of the Trans-Blot Turbo Transfer Pack (1704156, Bio-Rad Laboratories), using the Trans-Blot ® TurboTM Transfer System (Bio-Rad Laboratories) according to the manufacturer’s instructions. .. The blots were then fixed with a blocking solution of 1% casein in PBS (1610783, Bio-Rad Laboratories) and incubated for 2.5 h with specific primary antibodies—rabbit antibodies Atg5, Atg7, and LC3A/B from the Autophagy Antibody Sampler kit (4445, Cell signaling Technology, Danvers, MA, USA) and mouse antibody β-actin (8H10D10, Cell signaling Technology). .. This was followed by washing and 1 h incubation with a secondary horseradish peroxidase (HRP)-conjugated goat anti-rabbit antibody from the aforementioned kit or horse anti-mouse (7076P2, Cell signaling Technology) for the respective primary antibodies and washing again.

    Article Title: Sub-Immunosuppressive Tacrolimus Ameliorates Amyloid-Beta and Tau Pathology in 3xTg-AD Mice.
    Article Snippet: Proteins were then separated by electrophoresis on 4–15% Mini-PROTEAN® TGXTM Precast Protein Gels (catalog #4561086, Bio-Rad, Hercules, CA, USA) and transferred to Immobilon-P membranes (Millipore, St. Louis, MO, USA) for 1 h at 95 V at 4 ◦C. .. Membrane blocking was performed using 3% bovine serum albumin and Tris-buffered saline/0.1% Tween-20 (TBS-T) for 1 h at RT, followed by overnight incubation at 4 ◦C with primary antibodies: anti-tau13 (1:1000, catalog #835204, RRID:AB_2728543, BioLegend, San Diego, CA, USA), anti-phospho-tau (Ser202/Thr205; AT8) (1:1000, catalog # MN1020, RRID:AB_223647, Invitrogen, Carlsbad, CA, USA), anti-post synaptic density 95 (PSD95) (1:2000, catalog #3450, RRID:AB_2292883, Cell Signaling Technology, Danvers, MA, USA), anti-α-actinin (1:1000, catalog #3134, Cell Signaling Technology), anti-mammalian target of rapamycin (mTOR) (1:1000, catalog #2972, RRID:AB_330978, Cell Signaling Technology, Danvers, MA, USA), anti-phosphomTOR (Ser2448) (1:1000, catalog #2971, RRID:AB_330970, Cell Signaling Technology, Danvers, MA, USA), anti-70-kDa ribosomal protein S6 kinase (p70S6K) (1:1000, catalog #2708, RRID:AB_390722, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-p70S6K (Thr389) (1:1000, catalog# 9234, RRID:AB_2269803, Cell Signaling Technology, Danvers, MA, USA), anti-sequestosome-1 (p62) (1:1000, catalog #ab56416, RRID:AB_945626, Abcam), anticAMP response element binding (CREB) protein (1:1000, catalog #9104, RRID:AB_490881, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-CREB (Ser133) (1:1000, catalog# 9196, RRID:AB_331275, Cell Signaling Technology, Danvers, MA, USA), autophagy antibody sampler kit including Beclin-1, Atg5, Atg16 L1, Atg7, Atg3, LC3 A/B (1:1000, catalog# 4445, Cell Signaling Technology, Danvers, MA, USA), and anti-β-actin (1:50,000, catalog #A1978, RRID:AB_476692, Sigma-Aldrich, St. Louis, MO, USA). .. Following incubation, membranes were washed three times in 1X TBST for 10 min each, then incubated for 1 h at RT with HRP-conjugated secondary antibodies: anti-rabbit IgG (1:5000, catalog #7074, RRID:AB_2099233, Cell Signaling Technology, Danvers, MA, USA) and anti-mouse IgG (1:5000 catalog #7076, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA).

    Incubation:

    Article Title: Polyprenylated Acylphloroglucinols from Hypericum rochelii and Hypericum olympicum —Cytotoxic Effects on Non-Tumorigenic Cell Lines and Antibacterial Potential
    Article Snippet: The separated proteins were transferred to a polyvinylidene difluoride (PVDF) membrane, a part of the Trans-Blot Turbo Transfer Pack (1704156, Bio-Rad Laboratories), using the Trans-Blot ® TurboTM Transfer System (Bio-Rad Laboratories) according to the manufacturer’s instructions. .. The blots were then fixed with a blocking solution of 1% casein in PBS (1610783, Bio-Rad Laboratories) and incubated for 2.5 h with specific primary antibodies—rabbit antibodies Atg5, Atg7, and LC3A/B from the Autophagy Antibody Sampler kit (4445, Cell signaling Technology, Danvers, MA, USA) and mouse antibody β-actin (8H10D10, Cell signaling Technology). .. This was followed by washing and 1 h incubation with a secondary horseradish peroxidase (HRP)-conjugated goat anti-rabbit antibody from the aforementioned kit or horse anti-mouse (7076P2, Cell signaling Technology) for the respective primary antibodies and washing again.

    Article Title: Sub-Immunosuppressive Tacrolimus Ameliorates Amyloid-Beta and Tau Pathology in 3xTg-AD Mice.
    Article Snippet: Proteins were then separated by electrophoresis on 4–15% Mini-PROTEAN® TGXTM Precast Protein Gels (catalog #4561086, Bio-Rad, Hercules, CA, USA) and transferred to Immobilon-P membranes (Millipore, St. Louis, MO, USA) for 1 h at 95 V at 4 ◦C. .. Membrane blocking was performed using 3% bovine serum albumin and Tris-buffered saline/0.1% Tween-20 (TBS-T) for 1 h at RT, followed by overnight incubation at 4 ◦C with primary antibodies: anti-tau13 (1:1000, catalog #835204, RRID:AB_2728543, BioLegend, San Diego, CA, USA), anti-phospho-tau (Ser202/Thr205; AT8) (1:1000, catalog # MN1020, RRID:AB_223647, Invitrogen, Carlsbad, CA, USA), anti-post synaptic density 95 (PSD95) (1:2000, catalog #3450, RRID:AB_2292883, Cell Signaling Technology, Danvers, MA, USA), anti-α-actinin (1:1000, catalog #3134, Cell Signaling Technology), anti-mammalian target of rapamycin (mTOR) (1:1000, catalog #2972, RRID:AB_330978, Cell Signaling Technology, Danvers, MA, USA), anti-phosphomTOR (Ser2448) (1:1000, catalog #2971, RRID:AB_330970, Cell Signaling Technology, Danvers, MA, USA), anti-70-kDa ribosomal protein S6 kinase (p70S6K) (1:1000, catalog #2708, RRID:AB_390722, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-p70S6K (Thr389) (1:1000, catalog# 9234, RRID:AB_2269803, Cell Signaling Technology, Danvers, MA, USA), anti-sequestosome-1 (p62) (1:1000, catalog #ab56416, RRID:AB_945626, Abcam), anticAMP response element binding (CREB) protein (1:1000, catalog #9104, RRID:AB_490881, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-CREB (Ser133) (1:1000, catalog# 9196, RRID:AB_331275, Cell Signaling Technology, Danvers, MA, USA), autophagy antibody sampler kit including Beclin-1, Atg5, Atg16 L1, Atg7, Atg3, LC3 A/B (1:1000, catalog# 4445, Cell Signaling Technology, Danvers, MA, USA), and anti-β-actin (1:50,000, catalog #A1978, RRID:AB_476692, Sigma-Aldrich, St. Louis, MO, USA). .. Following incubation, membranes were washed three times in 1X TBST for 10 min each, then incubated for 1 h at RT with HRP-conjugated secondary antibodies: anti-rabbit IgG (1:5000, catalog #7074, RRID:AB_2099233, Cell Signaling Technology, Danvers, MA, USA) and anti-mouse IgG (1:5000 catalog #7076, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA).

    Western Blot:

    Article Title: MT1H inhibits the growth of gastric cancer by regulating SLC6A19/TTC39B/ADM2 and activating p53-dependent autophagy.
    Article Snippet: The ECL (Thermo Fisher Scientific) mixture was added to the membrane, and the images were visualized, adjusted, and captured with the Bio-Rad ChemiDocTM XRS + system (Bio-Rad, Hercules, CA, USA). .. For immunoblotting, LSD1 (2184), NF-κB p65 (8242T), RelB (4922T), Erk1/2 (9102), p-Erk1/2 (Thr202/Tyr204) (C4370S), and the Human Reactive Cell Death and Autophagy Antibody Sampler Kit (42867) were purchased from Cell Signaling Technology. .. Anti-Flag (F1804) and anti-HA (H6908) antibodies were obtained from Sigma‒ Aldrich.

    Article Title: MT1H inhibits the growth of gastric cancer by regulating SLC6A19/TTC39B/ADM2 and activating p53-dependent autophagy
    Article Snippet: The ECL (Thermo Fisher Scientific) mixture was added to the membrane, and the images were visualized, adjusted, and captured with the Bio-Rad ChemiDocTM XRS + system (Bio-Rad, Hercules, CA, USA). .. For immunoblotting, LSD1 (2184), NF-κB p65 (8242T), RelB (4922T), Erk1/2 (9102), p-Erk1/2 (Thr202/Tyr204) (C4370S), and the Human Reactive Cell Death and Autophagy Antibody Sampler Kit (42867) were purchased from Cell Signaling Technology. ..

    Membrane:

    Article Title: Sub-Immunosuppressive Tacrolimus Ameliorates Amyloid-Beta and Tau Pathology in 3xTg-AD Mice.
    Article Snippet: Proteins were then separated by electrophoresis on 4–15% Mini-PROTEAN® TGXTM Precast Protein Gels (catalog #4561086, Bio-Rad, Hercules, CA, USA) and transferred to Immobilon-P membranes (Millipore, St. Louis, MO, USA) for 1 h at 95 V at 4 ◦C. .. Membrane blocking was performed using 3% bovine serum albumin and Tris-buffered saline/0.1% Tween-20 (TBS-T) for 1 h at RT, followed by overnight incubation at 4 ◦C with primary antibodies: anti-tau13 (1:1000, catalog #835204, RRID:AB_2728543, BioLegend, San Diego, CA, USA), anti-phospho-tau (Ser202/Thr205; AT8) (1:1000, catalog # MN1020, RRID:AB_223647, Invitrogen, Carlsbad, CA, USA), anti-post synaptic density 95 (PSD95) (1:2000, catalog #3450, RRID:AB_2292883, Cell Signaling Technology, Danvers, MA, USA), anti-α-actinin (1:1000, catalog #3134, Cell Signaling Technology), anti-mammalian target of rapamycin (mTOR) (1:1000, catalog #2972, RRID:AB_330978, Cell Signaling Technology, Danvers, MA, USA), anti-phosphomTOR (Ser2448) (1:1000, catalog #2971, RRID:AB_330970, Cell Signaling Technology, Danvers, MA, USA), anti-70-kDa ribosomal protein S6 kinase (p70S6K) (1:1000, catalog #2708, RRID:AB_390722, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-p70S6K (Thr389) (1:1000, catalog# 9234, RRID:AB_2269803, Cell Signaling Technology, Danvers, MA, USA), anti-sequestosome-1 (p62) (1:1000, catalog #ab56416, RRID:AB_945626, Abcam), anticAMP response element binding (CREB) protein (1:1000, catalog #9104, RRID:AB_490881, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-CREB (Ser133) (1:1000, catalog# 9196, RRID:AB_331275, Cell Signaling Technology, Danvers, MA, USA), autophagy antibody sampler kit including Beclin-1, Atg5, Atg16 L1, Atg7, Atg3, LC3 A/B (1:1000, catalog# 4445, Cell Signaling Technology, Danvers, MA, USA), and anti-β-actin (1:50,000, catalog #A1978, RRID:AB_476692, Sigma-Aldrich, St. Louis, MO, USA). .. Following incubation, membranes were washed three times in 1X TBST for 10 min each, then incubated for 1 h at RT with HRP-conjugated secondary antibodies: anti-rabbit IgG (1:5000, catalog #7074, RRID:AB_2099233, Cell Signaling Technology, Danvers, MA, USA) and anti-mouse IgG (1:5000 catalog #7076, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA).

    Saline:

    Article Title: Sub-Immunosuppressive Tacrolimus Ameliorates Amyloid-Beta and Tau Pathology in 3xTg-AD Mice.
    Article Snippet: Proteins were then separated by electrophoresis on 4–15% Mini-PROTEAN® TGXTM Precast Protein Gels (catalog #4561086, Bio-Rad, Hercules, CA, USA) and transferred to Immobilon-P membranes (Millipore, St. Louis, MO, USA) for 1 h at 95 V at 4 ◦C. .. Membrane blocking was performed using 3% bovine serum albumin and Tris-buffered saline/0.1% Tween-20 (TBS-T) for 1 h at RT, followed by overnight incubation at 4 ◦C with primary antibodies: anti-tau13 (1:1000, catalog #835204, RRID:AB_2728543, BioLegend, San Diego, CA, USA), anti-phospho-tau (Ser202/Thr205; AT8) (1:1000, catalog # MN1020, RRID:AB_223647, Invitrogen, Carlsbad, CA, USA), anti-post synaptic density 95 (PSD95) (1:2000, catalog #3450, RRID:AB_2292883, Cell Signaling Technology, Danvers, MA, USA), anti-α-actinin (1:1000, catalog #3134, Cell Signaling Technology), anti-mammalian target of rapamycin (mTOR) (1:1000, catalog #2972, RRID:AB_330978, Cell Signaling Technology, Danvers, MA, USA), anti-phosphomTOR (Ser2448) (1:1000, catalog #2971, RRID:AB_330970, Cell Signaling Technology, Danvers, MA, USA), anti-70-kDa ribosomal protein S6 kinase (p70S6K) (1:1000, catalog #2708, RRID:AB_390722, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-p70S6K (Thr389) (1:1000, catalog# 9234, RRID:AB_2269803, Cell Signaling Technology, Danvers, MA, USA), anti-sequestosome-1 (p62) (1:1000, catalog #ab56416, RRID:AB_945626, Abcam), anticAMP response element binding (CREB) protein (1:1000, catalog #9104, RRID:AB_490881, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-CREB (Ser133) (1:1000, catalog# 9196, RRID:AB_331275, Cell Signaling Technology, Danvers, MA, USA), autophagy antibody sampler kit including Beclin-1, Atg5, Atg16 L1, Atg7, Atg3, LC3 A/B (1:1000, catalog# 4445, Cell Signaling Technology, Danvers, MA, USA), and anti-β-actin (1:50,000, catalog #A1978, RRID:AB_476692, Sigma-Aldrich, St. Louis, MO, USA). .. Following incubation, membranes were washed three times in 1X TBST for 10 min each, then incubated for 1 h at RT with HRP-conjugated secondary antibodies: anti-rabbit IgG (1:5000, catalog #7074, RRID:AB_2099233, Cell Signaling Technology, Danvers, MA, USA) and anti-mouse IgG (1:5000 catalog #7076, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA).

    Binding Assay:

    Article Title: Sub-Immunosuppressive Tacrolimus Ameliorates Amyloid-Beta and Tau Pathology in 3xTg-AD Mice.
    Article Snippet: Proteins were then separated by electrophoresis on 4–15% Mini-PROTEAN® TGXTM Precast Protein Gels (catalog #4561086, Bio-Rad, Hercules, CA, USA) and transferred to Immobilon-P membranes (Millipore, St. Louis, MO, USA) for 1 h at 95 V at 4 ◦C. .. Membrane blocking was performed using 3% bovine serum albumin and Tris-buffered saline/0.1% Tween-20 (TBS-T) for 1 h at RT, followed by overnight incubation at 4 ◦C with primary antibodies: anti-tau13 (1:1000, catalog #835204, RRID:AB_2728543, BioLegend, San Diego, CA, USA), anti-phospho-tau (Ser202/Thr205; AT8) (1:1000, catalog # MN1020, RRID:AB_223647, Invitrogen, Carlsbad, CA, USA), anti-post synaptic density 95 (PSD95) (1:2000, catalog #3450, RRID:AB_2292883, Cell Signaling Technology, Danvers, MA, USA), anti-α-actinin (1:1000, catalog #3134, Cell Signaling Technology), anti-mammalian target of rapamycin (mTOR) (1:1000, catalog #2972, RRID:AB_330978, Cell Signaling Technology, Danvers, MA, USA), anti-phosphomTOR (Ser2448) (1:1000, catalog #2971, RRID:AB_330970, Cell Signaling Technology, Danvers, MA, USA), anti-70-kDa ribosomal protein S6 kinase (p70S6K) (1:1000, catalog #2708, RRID:AB_390722, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-p70S6K (Thr389) (1:1000, catalog# 9234, RRID:AB_2269803, Cell Signaling Technology, Danvers, MA, USA), anti-sequestosome-1 (p62) (1:1000, catalog #ab56416, RRID:AB_945626, Abcam), anticAMP response element binding (CREB) protein (1:1000, catalog #9104, RRID:AB_490881, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-CREB (Ser133) (1:1000, catalog# 9196, RRID:AB_331275, Cell Signaling Technology, Danvers, MA, USA), autophagy antibody sampler kit including Beclin-1, Atg5, Atg16 L1, Atg7, Atg3, LC3 A/B (1:1000, catalog# 4445, Cell Signaling Technology, Danvers, MA, USA), and anti-β-actin (1:50,000, catalog #A1978, RRID:AB_476692, Sigma-Aldrich, St. Louis, MO, USA). .. Following incubation, membranes were washed three times in 1X TBST for 10 min each, then incubated for 1 h at RT with HRP-conjugated secondary antibodies: anti-rabbit IgG (1:5000, catalog #7074, RRID:AB_2099233, Cell Signaling Technology, Danvers, MA, USA) and anti-mouse IgG (1:5000 catalog #7076, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA).

    other:

    Article Title: Ubiquitination of gasdermin D N-terminal domain directs its membrane translocation and pore formation during pyroptosis.
    Article Snippet: For ELISA, mouse IL-1β kit (EM0109), mouse IL-18 kit (EM1158), mouse TNFα kit (EM0183), and mouse HMGB1 kit (EM0382) were obtained from Finetest.

    Article Title: Ubiquitination of gasdermin D N-terminal domain directs its membrane translocation and pore formation during pyroptosis
    Article Snippet: For ELISA, mouse IL-1β kit (EM0109), mouse IL-18 kit (EM1158), mouse TNFα kit (EM0183), and mouse HMGB1 kit (EM0382) were obtained from Finetest.



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    Image Search Results


    Crucial RBP gene NOP58 in the progression of EwS. ( A ). The PPI network of top 10 genes in the 179 candidate genes. ( B ). The top 15 genes screened out by P value of univariate COX regression analysis on 179 candidate genes. ( C ). The NOP58’s expression of tumor group and normal group in GSE68776 . ( D ). The NOP58’s expression of tumor group and normal group in GSE17679 . ( E ) Western blot and ( F ) qRT-PCR analysis of NOP58 expression in normal and EwS cell lines

    Journal: Hereditas

    Article Title: RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma

    doi: 10.1186/s41065-025-00440-5

    Figure Lengend Snippet: Crucial RBP gene NOP58 in the progression of EwS. ( A ). The PPI network of top 10 genes in the 179 candidate genes. ( B ). The top 15 genes screened out by P value of univariate COX regression analysis on 179 candidate genes. ( C ). The NOP58’s expression of tumor group and normal group in GSE68776 . ( D ). The NOP58’s expression of tumor group and normal group in GSE17679 . ( E ) Western blot and ( F ) qRT-PCR analysis of NOP58 expression in normal and EwS cell lines

    Article Snippet: Secondary antibodies, anti-rabbit IgG HRP-linked antibody for NOP58 (7075, 1:5000 dilution) and anti-mouse IgG HRP-linked antibody for β-actin (7076, 1:5000 dilution), were purchased from Cell Signaling Technology (CST, Danvers, MA, US).

    Techniques: Expressing, Western Blot, Quantitative RT-PCR

    Pan-cancer analysis of NOP58. ( A ). The expression of NOP58 in various normal tissues in HPA database. ( B ). Subcellular localization of NOP58 in osteosarcoma cell line U2OS in HPA database. ( C ). The expression of NOP58 in different tissues in GEPIA database. ( D ). NOP58 expression in different cell lines in CCLE database. * meant p was smaller or equaled 0.05, ** meant p was smaller or equaled 0.01, *** meant p was smaller or equaled 0.001, **** meant p was smaller or equaled 0.0001

    Journal: Hereditas

    Article Title: RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma

    doi: 10.1186/s41065-025-00440-5

    Figure Lengend Snippet: Pan-cancer analysis of NOP58. ( A ). The expression of NOP58 in various normal tissues in HPA database. ( B ). Subcellular localization of NOP58 in osteosarcoma cell line U2OS in HPA database. ( C ). The expression of NOP58 in different tissues in GEPIA database. ( D ). NOP58 expression in different cell lines in CCLE database. * meant p was smaller or equaled 0.05, ** meant p was smaller or equaled 0.01, *** meant p was smaller or equaled 0.001, **** meant p was smaller or equaled 0.0001

    Article Snippet: Secondary antibodies, anti-rabbit IgG HRP-linked antibody for NOP58 (7075, 1:5000 dilution) and anti-mouse IgG HRP-linked antibody for β-actin (7076, 1:5000 dilution), were purchased from Cell Signaling Technology (CST, Danvers, MA, US).

    Techniques: Expressing

    The results of immune infiltration analysis. ( A ). The relative content of 22 kinds of immune infiltrating cells in NOP58 high and low expression groups in GSE17618 . ( B ). The CIBERSORT algorithm was employed to evaluate the immune infiltration of 22 kinds of immune cells among the samples in NOP58 high and low expression groups in the GSE17618 dataset. ( C ). The xCell algorithm was conducted to assess the immune infiltration of 36 kinds of immune cells among the samples in NOP58 high and low expression groups in the GSE68776 dataset. ( D ). Expression of PDCD1 between NOP58 high and low expression groups in the GSE17618 dataset. ( E , F , G ). The stroma, immune, and ESTIMATE scores between NOP58 high and low expression groups in the GSE68776 dataset. * means P < 0.05; ** means P < 0.01

    Journal: Hereditas

    Article Title: RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma

    doi: 10.1186/s41065-025-00440-5

    Figure Lengend Snippet: The results of immune infiltration analysis. ( A ). The relative content of 22 kinds of immune infiltrating cells in NOP58 high and low expression groups in GSE17618 . ( B ). The CIBERSORT algorithm was employed to evaluate the immune infiltration of 22 kinds of immune cells among the samples in NOP58 high and low expression groups in the GSE17618 dataset. ( C ). The xCell algorithm was conducted to assess the immune infiltration of 36 kinds of immune cells among the samples in NOP58 high and low expression groups in the GSE68776 dataset. ( D ). Expression of PDCD1 between NOP58 high and low expression groups in the GSE17618 dataset. ( E , F , G ). The stroma, immune, and ESTIMATE scores between NOP58 high and low expression groups in the GSE68776 dataset. * means P < 0.05; ** means P < 0.01

    Article Snippet: Secondary antibodies, anti-rabbit IgG HRP-linked antibody for NOP58 (7075, 1:5000 dilution) and anti-mouse IgG HRP-linked antibody for β-actin (7076, 1:5000 dilution), were purchased from Cell Signaling Technology (CST, Danvers, MA, US).

    Techniques: Expressing

    NOP58’s clinical value in EwS. ( A ). Results of multivariate COX regression analysis. ( B ). Time-dependent ROC curve of NOP58’s prognostic value at 1, 3 and 5 years. ( C ). Kaplan-Meier (KM) survival analysis based on GEO-meta datasets merged by GSE17618 and GSE218758 datasets. ( D ). NOP58 expression between Alive and Dead groups. ( E ) GSEA results. * meant p was smaller or equaled 0.05, ** meant p was smaller or equaled 0.01

    Journal: Hereditas

    Article Title: RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma

    doi: 10.1186/s41065-025-00440-5

    Figure Lengend Snippet: NOP58’s clinical value in EwS. ( A ). Results of multivariate COX regression analysis. ( B ). Time-dependent ROC curve of NOP58’s prognostic value at 1, 3 and 5 years. ( C ). Kaplan-Meier (KM) survival analysis based on GEO-meta datasets merged by GSE17618 and GSE218758 datasets. ( D ). NOP58 expression between Alive and Dead groups. ( E ) GSEA results. * meant p was smaller or equaled 0.05, ** meant p was smaller or equaled 0.01

    Article Snippet: Secondary antibodies, anti-rabbit IgG HRP-linked antibody for NOP58 (7075, 1:5000 dilution) and anti-mouse IgG HRP-linked antibody for β-actin (7076, 1:5000 dilution), were purchased from Cell Signaling Technology (CST, Danvers, MA, US).

    Techniques: Expressing

    Knockdown of NOP58 influenced the proliferation of EwS cells. ( A , B ). An immunofluorescence assay was performed to detect the localization of the NOP58 protein in SK-N-MC and A673 cells using microscopy. ( C , D ). SK-N-MC and A673 cells were transfected with sh Ctrl, sh1 NOP58 and sh2 NOP58. Western blot and qRT-PCR assays were conducted to evaluate NOP58 expression in transfected cells. ( E , F ). CCK-8 assay was performed to assess cell proliferation. *** meant p was smaller or equaled 0.001; **** meant p was smaller or equaled 0.0001

    Journal: Hereditas

    Article Title: RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma

    doi: 10.1186/s41065-025-00440-5

    Figure Lengend Snippet: Knockdown of NOP58 influenced the proliferation of EwS cells. ( A , B ). An immunofluorescence assay was performed to detect the localization of the NOP58 protein in SK-N-MC and A673 cells using microscopy. ( C , D ). SK-N-MC and A673 cells were transfected with sh Ctrl, sh1 NOP58 and sh2 NOP58. Western blot and qRT-PCR assays were conducted to evaluate NOP58 expression in transfected cells. ( E , F ). CCK-8 assay was performed to assess cell proliferation. *** meant p was smaller or equaled 0.001; **** meant p was smaller or equaled 0.0001

    Article Snippet: Secondary antibodies, anti-rabbit IgG HRP-linked antibody for NOP58 (7075, 1:5000 dilution) and anti-mouse IgG HRP-linked antibody for β-actin (7076, 1:5000 dilution), were purchased from Cell Signaling Technology (CST, Danvers, MA, US).

    Techniques: Knockdown, Immunofluorescence, Microscopy, Transfection, Western Blot, Quantitative RT-PCR, Expressing, CCK-8 Assay

    Knockdown of NOP58 influenced the migration of EwS cells. SK-N-MC and A673 cells were transfected with sh Ctrl, sh1 NOP58 and sh2 NOP58. ( A , B ). Transwell migration and ( C , D ) wound healing assays were utilized to determine cell migration. * meant p was smaller or equaled 0.05, ** meant p was smaller or equaled 0.01, *** meant p was smaller or equaled 0.001

    Journal: Hereditas

    Article Title: RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma

    doi: 10.1186/s41065-025-00440-5

    Figure Lengend Snippet: Knockdown of NOP58 influenced the migration of EwS cells. SK-N-MC and A673 cells were transfected with sh Ctrl, sh1 NOP58 and sh2 NOP58. ( A , B ). Transwell migration and ( C , D ) wound healing assays were utilized to determine cell migration. * meant p was smaller or equaled 0.05, ** meant p was smaller or equaled 0.01, *** meant p was smaller or equaled 0.001

    Article Snippet: Secondary antibodies, anti-rabbit IgG HRP-linked antibody for NOP58 (7075, 1:5000 dilution) and anti-mouse IgG HRP-linked antibody for β-actin (7076, 1:5000 dilution), were purchased from Cell Signaling Technology (CST, Danvers, MA, US).

    Techniques: Knockdown, Migration, Transfection

    Knockdown of NOP58 influenced the apoptosis of EwS cells in vitro and tumor growth in vivo. ( A , B , C , D ). SK-N-MC and A673 cells were transfected with sh Ctrl, sh1 NOP58 and sh2 NOP58. Cell apoptosis was detected by TUNEL staining assay and flow cytometry. ( E , F ). Cell cycle distribution was determined by flow cytometry. ( G ). In vivo tumor growth of mice injected subcutaneously with NOP58-knockdown SK-N-MC cells. * meant p was smaller or equaled 0.05, ** meant p was smaller or equaled 0.01, *** meant p was smaller or equaled 0.001

    Journal: Hereditas

    Article Title: RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma

    doi: 10.1186/s41065-025-00440-5

    Figure Lengend Snippet: Knockdown of NOP58 influenced the apoptosis of EwS cells in vitro and tumor growth in vivo. ( A , B , C , D ). SK-N-MC and A673 cells were transfected with sh Ctrl, sh1 NOP58 and sh2 NOP58. Cell apoptosis was detected by TUNEL staining assay and flow cytometry. ( E , F ). Cell cycle distribution was determined by flow cytometry. ( G ). In vivo tumor growth of mice injected subcutaneously with NOP58-knockdown SK-N-MC cells. * meant p was smaller or equaled 0.05, ** meant p was smaller or equaled 0.01, *** meant p was smaller or equaled 0.001

    Article Snippet: Secondary antibodies, anti-rabbit IgG HRP-linked antibody for NOP58 (7075, 1:5000 dilution) and anti-mouse IgG HRP-linked antibody for β-actin (7076, 1:5000 dilution), were purchased from Cell Signaling Technology (CST, Danvers, MA, US).

    Techniques: Knockdown, In Vitro, In Vivo, Transfection, TUNEL Assay, Staining, Flow Cytometry, Injection